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CCNT1 Antibody (Center)
Description
Research Area
Images & Validation
−| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB - 1:1000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 80685 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |

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All lanes: Anti-CCNT1 Antibody (Center) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: K562 whole cell lysate. Lane 6: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 81 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

CCNT1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

CCNT1 Antibody (Center) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the CCNT1 antibody detected the CCNT1 protein (arrow).

CCNT1 Antibody (Center) western blot analysis in mouse testis tissue lysates (35 ug/lane). This demonstrates the CCNT1 antibody detected the CCNT1 protein (arrow).
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Protocol Information
CCNT1 Antibody (Center) (orb1938808)
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