CD10 Antibody / Neutral endopeptidase 24.11 / Neprilysin

SKU: orb1823561

Description

Recognizes a 100kDa glycoprotein, identified as CD10, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Images & Validation

−

Item 1 of 3

Tested Applications	WB
Dilution Range	Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the CD10 antibody should be determined by the researcher.

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	MME/8658R
Immunogen	A recombinant human CD10 protein fragment (within amino acids 1-500) was used as the immunogen for the CD10 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

CD10 Antibody / Neutral endopeptidase 24.11 / Neprilysin

Western blot testing of human kidney tissue lysate with CD10 antibody. Routinely visualized at ~100 kDa.

SDS-PAGE analysis of purified, BSA-free CD10 antibody (clone MME/8658R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P08473

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

Find More Protocols