CD10 Rabbit Polyclonal Antibody

SKU: orb10284

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Description

Images & Validation

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Item 1 of 5

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human, Rat
Predicted Reactivity	Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CD10 (490-530/750aa)
Target	MME
Molecular Weight	100 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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NEP_HUMAN; Neprilysin; MME; membrane metalloendopeptidase; NEP; SFE; CD10; CALLA; CMT2T; SCA43; Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; Neutral endopeptidase; Skin fibroblast elastase;

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Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD10) Polyclonal Antibody, Unconjugated (orb10284) at 1:25 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD10) Polyclonal Antibody, Unconjugated (orb10284) at 1:25 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD10) Polyclonal Antibody, Unconjugated (orb10284) at 1:25 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Rat Kidney tissue lysates, Lane 2: Human RAMOS cell lysates, Primary: Anti-CD10 (orb10284) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 82 kDa, Observed band size: 100 kDa.

Sample: Raji (Human) Cell Lysate at 30 ug, Primary: Anti-CD10 (orb10284) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 82 kD, Observed band size: 82 kD.

Quick Database Links

Gene Symbol

MME

UniProt

P08473

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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