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CD11a Recombinant Mouse Monoclonal Antibody
SKU: orb1152076
Featured
Description
Research Area
Cell Biology, Erythropoiesis, Immune System, Immunology, Innate Immunity, Mast Cells, Natural Killer Cell Lineage, Stem Cell Research, Stem Cells
Images & Validation
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| Tested Applications | FC, ICC |
|---|---|
| Dilution Range | ICC/IF=1:100-500, Flow-Cyt=1ug/Test |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | A13C2 |
| Immunogen | KLH conjugated synthetic peptide derived from human CD11a |
| Target | ITGAL |
| Molecular Weight | 126 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD11a; Integrin alpha-L; Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA-1A; Leukocyte function-associated molecule 1 alpha chain; Lymphocyte antigen 15; Ly-15; CD11 antigen-like family member A; CD_antigen; ITAL_HUMAN.
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Blank control: Raji. Primary Antibody (green line): Mouse Anti-CD11a antibody (orb1152076), Dilution: 1:100, Secondary Antibody: Goat anti-mouse IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Cell line: Jurkat, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% Triton X-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152076.
Specimen: PBMC, Fixative: Unfixed, Permeabilization: None, Primary Ab Dilution: 1:100, Secondary Ab: Goat anti Mouse IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Mouse monoclonal IgG1 (Blue line). Comment: Line red is the positive signal for orb1152076.
Quick Database Links
Gene Symbol
ITGAL
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
FC
Flow Cytometry
ICC
Immunocytochemistry
CD11a Recombinant Mouse Monoclonal Antibody (orb1152076)
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