CD11b Rabbit Polyclonal Antibody

SKU: orb1294243

Description

Rabbit polyclonal antibody to CD11b

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 4

Tested Applications	IHC, WB
Dilution Range	Western Blot 1:500 - 1:1000, Immunofluorescence 1:200 – 1:300, Immunohistochemistry 1: 100
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / Extracellular domain corresponding to Human CD11b
Target	CD11b / ITGAM human specific
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Anti-CD11b / ITGAM antibody - at 1/500 dilution. Lysates at 50 µg per lane. NC membrane was then blocked for an hour before being incubated with orb1294243 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD11b with orb1294243 at 1/200.

Immunohistochemistry (frozen sections), Sample: Hippocampus, Antigen retrieval: 10 mM Citrate, pH 6.0, Fixative: Paraformaldehyde.

Quick Database Links

Gene Symbol

CD11b / ITGAM human specific

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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