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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution Range | WB: 1:500-2000, IHC-P: 1:50-100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 179-433 of human CD133 (NP_006008.1). |
| Molecular Weight | 120kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti-AC133 antibody, gen AC133 antibody, anti-CD133 antibody, anti-CD133 Partial Recombinant Protein antibody, anti-CORD12 antibody, anti-Hematopoietic stem cellgen antibody, anti-hProminin antibody, anti-MCDR2 antibody, anti-MSTP061 antibody, anti-PROM antibody, anti-PROM1 antibody, anti-Prominin I antibody, anti-Prominin like protein 1 precursor antibody, anti-Prominin mouse like 1 antibody, anti-prominin1 antibody, anti-PROML1 antibody, anti-RP41 antibody, anti-STGD4 antibody, anti-AC133 antibody, anti-Antigen AC133 antibody, anti-Hematopoietic stem cell antigen antibody, anti-hProminin antibody, anti-PROM1 antibody, anti-Prominin I antibody, anti-Prominin like protein 1 precursor antibody, anti-Prominin mouse like 1 antibody, anti-prominin1 antibody, anti-PROML1 antibody, anti-CD133 antibody, anti-CORD12 antibody, anti-MCDR2 antibody, anti-MSTP061 antibody, anti-PROML1 antibody, anti-RP41 antibody, anti-STGD4.
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Immunofluorescence analysis of NIH/3T3 cells using CD133 Rabbit pAb (orb129549) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using CD133 Rabbit pAb (orb129549) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of lysates from HCT116 cells, using CD133 Rabbit pAb (orb129549) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of various lysates using CD133 Rabbit pAb (orb129549) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Filter by Applications
Filter by Species
Singer, Donald et al. Prominin‐1 controls stem cell activation by orchestrating ciliary dynamics The EMBO Journal, e99845 (2018)
Applications
IF
Reactivity
Mouse
CD133 Rabbit pAb (orb129549)
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