TNFSF9 Antibody (Biotin)

SKU: orb751086

Description

Mouse monoclonal antibody against TNFSF9 conjugated to Biotin

Research Area

Immunology & Inflammation, Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	FC, IF, IHC
Dilution Range	Flow Cytometry (2-4ug/million cells in 0.1ml); Immunofluorescence (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Reactivity	Human
Application Notes	Positive Control: Raji, 293T or HEK293 cells. Human renal cell carcinoma or lung carcinoma.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	CD137L/1547
Immunogen	Recombinant full-length human CD137L protein
Target	TNFSF9
Source/Expression System	Bioreactor
Molecular Weight	27kDa
Purification	Protein A
Conjugation	Biotin

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Buffer/Preservatives	Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Concentration	Purified Ab conjugated to Biotin
Disclaimer	For research use only

Alternative Names

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Anti-4-1BB ligand antibody, anti-4-1BBL antibody, anti-CD137L antibody, anti-ILA ligand (TNF related) antibody, anti-Ly63l antibody, anti-Tumor necrosis factor ligand 5A (TNLG5A) antibody, anti-Tumor necrosis factor ligand superfamily member 9 (TNFSF9) antibody

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Flow Cytometric Analysis of PFA-fixed HEK293 cells using CD137L-Monospecific Mouse Monoclonal Antibody (CD137L/1547) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD137L-Monospecific Mouse Monoclonal Antibody (CD137L/1547).

SDS-PAGE Analysis Purified CD137L-Monospecific Mouse Monoclonal Antibody (CD137L/1547). Confirmation of Purity and Integrity of Antibody.

Quick Database Links

Gene Symbol

TNFSF9

UniProt

P41273

NCBI Gene ID

8744

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence