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CD138 Rabbit Polyclonal Antibody
SKU: orb1294300
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:500, Immunofluorescence 1:200, Immunohistochemistry (Paraffin) 1:100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human CD138 / Syndecan-1 |
| Target | CD138 / Syndecan-1 (IHC prefer) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Disclaimer | For research use only |
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All lanes: anti-CD138 / Syndecan-1 antibody at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294300 overnight at 4°C.
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294300 anti-CD138 / Syndecan-1 antibody (red) at 1:200 and 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD138 / Syndecan-1 with orb1294300 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD138 / Syndecan-1 with orb1294300 at 1/100.
Quick Database Links
Gene Symbol
CD138 / Syndecan-1 (IHC prefer)
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
CD138 Rabbit Polyclonal Antibody (orb1294300)
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