Recombinant CD147 Antibody / Basigin / Emmprin

SKU: orb2636029

Description

CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Images & Validation

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Tested Applications	ELISA, IHC-P, WB
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant CD147 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	BSG/9223R
Immunogen	Recombinant full-length human BSG protein was used as the immunogen for the recombinant CD147 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant CD147 Antibody / Basigin / Emmprin

Western blot testing of human HepG2 cell lysate with recombinant CD147 antibody (clone BSG/9223R). Expected molecular weight: 27-66 kDa depending on level of glycosylation.

IHC staining of FFPE human placenta tissue with recombinant CD147 antibody (clone BSG/9223R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE Analysis recombinant CD147 antibody (clone BSG/9223R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P35613

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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