Recombinant CD20 Antibody / Rabbit Monoclonal

SKU: orb386222

Description

CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells.

Research Area

Epigenetics

Images & Validation

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Tested Applications	FACS, IHC-P
Dilution Range	Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Titering of the recombinant CD20 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	IGEL/1497R
Immunogen	Full length human CD20 protein was used as the immunogen for this reccombinant CD20 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant CD20 Antibody / Rabbit Monoclonal

IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. Required HIER: steam section in pH9 10 mM Tris with 1 mM EDTA for 20 min.

Flow cytometry testing of human Raji cells with recombinant CD20 antibody (clone IGEL/1497R); Red = isotype control, Blue = recombinant CD20 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant CD20 antibody (clone IGEL/1497R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P11836

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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