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CD21 Recombinant Rabbit Monoclonal Antibody
SKU: orb1151979
Featured
Description
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| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | C12B9 |
| Immunogen | A synthesized peptide derived from human CD21 (1000-1033/1033aa) |
| Target | CR2 |
| Molecular Weight | 111 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−C3DR; CD 21; CD21 antigen; Complement C3d receptor; Complement component(3d/Epstein Barr virus) receptor 2; Complement component receptor 2; Complement receptor type 2; Cr 2; Cr2; EBV receptor; Epstein Barr virus receptor; CR2_HUMAN.
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Cell line: Raji, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:100, Primary incubation condition: 1 hour at room temperature, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1151979.
Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD21) Monoclonal Antibody, Unconjugated (orb1151979) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Human Raji cell lysates, Lane 2: Human Raji cell lysates, Primary: Anti-CD21 (orb1151979) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 111 kDa, Observed band size: 150 kDa.
Quick Database Links
Gene Symbol
CR2
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
CD21 Recombinant Rabbit Monoclonal Antibody (orb1151979)
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