Recombinant CD27 Antibody

SKU: orb1825874

Description

Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFkB and SAPK/JNK and induces apoptosis.

Images & Validation

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Tested Applications	IHC-P, WB
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant CD27 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	LPFS2/8607R
Immunogen	Recombinant human full-length protein was used as the immunogen for the recombinant CD27 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC staining of FFPE human tonsil tissue with recombinant CD27 antibody (clone LPFS2/8607R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CD27 antibody (clone LPFS2/8607R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P26842

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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