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CD274 Antibody
SKU: orb629783
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FC, IF, IHC, IP, WB |
|---|---|
| Dilution range | WB: 1:200-1:1000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | CD274 molecule |
| Target | CD274 |
| Molecular Weight | 50 kDa, 70 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Disclaimer | For research use only |
Alternative Names
−B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1, PD-L1/CD274, Programmed death ligand 1
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mouse heart tissue were subjected to SDS PAGE followed by western blot with orb629783 (PD-L1/CD274 Antibody) at dilution of 1:300.
Immunofluorescent analysis of HEK-293 cells using orb629783 (PD-L1/CD274 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).
IP Result of anti-PD-L1/CD274 (IP: orb629783, 4 ug; Detection: orb629783 1:1000) with mouse heart tissue lysate 4000 ug.
Immunohistochemistry of paraffin-embedded mouse heart tissue slide using orb629783 (PD-L1/CD274 Antibody) at dilution of 1:50.
Quick Database Links
Gene Symbol
CD274
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
CD274 Antibody (orb629783)
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