Recombinant CD38 Antibody

SKU: orb1151661

Description

Rabbit monoclonal antibody to CD38

Research Area

Epigenetics

Images & Validation

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Item 1 of 5

Tested Applications	IHC-P, WB
Dilution range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant CD38 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	CD38/6448R
Immunogen	A portion of amino acids 200-300 of human CD38 protein was used as the immunogen for the recombinant CD38 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Western blot testing of human Raji cell lysate using recombinant CD38 antibody (clone CD38/6448R). Expected molecular weight: 34-46 kDa depending on glycosylation level.

SDS-PAGE analysis of purified, BSA-free recombinant CD38 antibody (clone CD38/6448R) as confirmation of integrity and purity.

IHC staining of FFPE human tonsil tissue with recombinant CD38 antibody (clone CD38/6448R) at 2 ug/ml in PBS for 30 min RT. Strong cytoplasmic and membranous staining is observed. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue with recombinant CD38 antibody (clone CD38/6448R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate adenocarcinoma tissue with recombinant CD38 antibody (clone CD38/6448R) at 2 ug/ml in PBS for 30 min RT. Strong cytoplasmic and membranous staining observed. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P28907

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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