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CD4 Antibody
SKU: orb1239267
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Description
Images & Validation
−Item 1 of 8
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 9H5A8 |
| Immunogen | Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4. |
| Target | CD4 |
| Molecular Weight | Predicted: 51kDObserved: 51-57 kD (Post-modyfication: 2-4 N-linked glycosylations) |
| Purification | CD4 Monoclonal Antibody is immunoaffinity chromotography purified IgG. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−CD4 Antibody [9H5A8] : CD4mut
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Western Blot Validation with Human Recombinant Protein.Loading: 5ng (Lane 1), 25ng (Lane 2) and 100ng (Lane 3) of human CD4 recombinant protein. Antibodies: CD4 orb1239267 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Observed at around 27kD.
Western Blot Validation in Human Thymus Tissue Lysate.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239267 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Western Blot Validation in Human Jurkat Lysate.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239267 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Western Blot Validation in Human, Mouse and Rat Tissue Lysate.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239267 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Western Blot Validation in Human Cell Lines.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239267 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Western Blot Validation in Mouse Spleen Tissue Lysate.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239267 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Western Blot Validation in Rat Lung Tissue Lysate.Loading: 15 μg of lysates per lane. Antibodies: CD4 orb1239267 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
Immunohistochemistry Validation of CD4 in Human Thymus Tissue.Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-CD4 antibody (orb1239267) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD4 Antibody (orb1239267)
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