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CD4 Antibody (C-term)
SKU: orb2649321
Description
Images & Validation
−Item 1 of 5
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB - 1:2000 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Monkey |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 51111 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
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−- Item 1 of 3
CD4 Antibody [orb213686]
IF, IHC, WB
Canine, Human, Monkey, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
30 μl, 100 μl, 200 μl, 50 μl
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Western blot analysis of anti-CD4 Antibody (C-term) in mouse heart tissue lysates (35 ug/lane). CD4 (arrow) was detected using the purified Pab.
Flow cytometric analysis of CEM cells using CD4 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes: Anti-CD4 Antibody (C-term) at 1:2000 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 51 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human Tonsil tissue reacted with CD4 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human Tonsil tissue reacted with CD4 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
CD4 Antibody (C-term) (orb2649321)
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