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CD4 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | Blocking, ELISA, FC, IHC |
|---|---|
| Reactivity | Human, Primate |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | IgG4 lambda |
| Clone No. | CE9.1 (Clenoliximab) |
| Immunogen | Domain 1 of human CD4. |
| Target | CD4 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western Blot using anti-CD4 antibody CE9.1 (Clenolixim450). Human thyroid tumour samples were resolved on a 15% SDS PAGE gel and blots probed with the human IgG4-chimeric version of CE9.1 (orb1671990) at 1.5 ug/ml before detection using an anti-human IgG4 secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD4 is 51kDa (arrowhead). Here some non-specific bands can also be seen. orb1671990 successfully detected CD4 in human thyroid tissue samples.

Immunohistochemical staining of guinea pig skin using anti-CD4 antibody (CE9.1). ClenoliximAnti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue - at 40x magnification. The human IgG4-chimeric version of CE9.1 (Clenolixim92) was used to stain samples at a concentration of 3 ug/ml.
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CD4 Antibody (orb1671990)
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