CD40 Ligand Antibody / TRAP / CD154

SKU: orb606874

Description

CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.

Images & Validation

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Tested Applications	ELISA, FACS, IF, IHC-P
Dilution range	ELISA: order BSA/sodium azide-free format for coating,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the CD40 Ligand antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2b, kappa
Clone No.	CD40LG/2761
Immunogen	A portion of amino acids 108-261 from the human protein was used as the immunogen for the CD40 Ligand antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC testing of FFPE human spleen with CD40 Ligand antibody (clone CD40LG/2761). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD40 Ligand antibody (clone CD40LG/2761). These results demonstrate the foremost specificity of the CD40LG/2761 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human spleen with CD40 Ligand antibody (clone CD40LG/2761). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human tonsil with CD40 Ligand antibody (clone CD40LG/2761). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of paraformaldehyde-fixed human Jurkat cells with CD40 Ligand antibody (green, clone CD40LG/2761) and Reddot nuclear stain (red).

Flow testing of human Jurkat cells with CD40 Ligand antibody (blue) and isotype control (red).

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UniProt

P29965

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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