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CD44 Antibody / HCAM
SKU: orb1151854
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P |
|---|---|
| Dilution Range | Immunohistochemistry (FFPE): 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | Mouse IgG2a, kappa |
| Clone No. | rHCAM/6449 |
| Immunogen | Recombinant full-length human CD44 protein was used as the immunogen for the CD44 antibody. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC staining of FFPE human esophagus tissue with CD44 antibody (clone rHCAM/6449). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast tissue with CD44 antibody (clone rHCAM/6449). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using CD44 antibody (clone rHCAM/6449) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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CD44 Antibody / HCAM (orb1151854)
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