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CD45 (Intracellular) Antibody
SKU: orb1294366
Description
Images & Validation
−Item 1 of 13
| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1: 200-1:400 Flow Cytometry 1:50-1:100 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Intracellular domain corresponding to Human CD45 |
| Target | CD45 (Intracellular) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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All lanes: Anti-CD45 antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294366 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294366 anti-CD45 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294366 anti-CD45 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling CD45 with orb1294366 at 1/300.
Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling CD45 with orb1294366 at 1/300.
Immunohistochemical analysis of paraffin embedded Human Gastric cancer tissue labeling CD45 with orb1294366 at 1/300.
Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling CD45 with orb1294366 at 1/300.
Immunohistochemical analysis of paraffin embedded Human Lung cancer tissue labeling CD45 with orb1294366 at 1/300.
Immunohistochemical analysis of paraffin embedded Human Lymph node carcinoma labeling CD45 with orb1294366 at 1/300.
Immunohistochemical analysis of paraffin embedded Human Ovary cancer tissue labeling CD45 with orb1294366 at 1/300.
Immunohistochemical analysis of paraffin embedded Human Squamous cell carcinoma labeling CD45 with orb1294366 at 1/300.
Immunohistochemical analysis of paraffin embedded Human Tonsil cancer tissue labeling CD45 with orb1294366 at 1/300.
CD45 antibody, orb1294366, Cell: THP-1.
Quick Database Links
Gene Symbol
CD45 (Intracellular)
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
CD45 (Intracellular) Antibody (orb1294366)
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