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PTPRC Antibody
SKU: orb1252710
Description
Images & Validation
−Item 1 of 6
| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a, kappa |
| Clone No. | 158-4D3 |
| Immunogen | Stimulated human leukocytes were used as the immunogen for this CD45RA antibody. |
| Target | PTPRC |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Concentration | 0.2 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
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IHC staining of FFPE human tonsil with CD45RA antibody (clone 158-4D3). HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC staining of FFPE human spleen with CD45RA antibody (clone 158-4D3). HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
FACS surface staining of human PBMCs using CD45RA antibody (clone 158-4D3, red), and isotype control (green).
Flow cytometry testing of human Jurkat cells with CD45RA antibody (clone 158-4D3); Red = isotype control, Blue = CD45RA antibody.
Western blot testing of human spleen lysate with CD45RA antibody (clone 158-4D3). Expected molecular weight: 147-220 kDa depending on glycosylation level.
SDS-PAGE analysis of purified, BSA-free CD45RA antibody (clone 158-4D3) as confirmation of integrity and purity.
Quick Database Links
Gene Symbol
PTPRC
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
PTPRC Antibody (orb1252710)
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