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CD45RO Antibody
SKU: orb385805
Description
Research Area
Epigenetics, Infectious Diseases
Images & Validation
−Item 1 of 2
| Tested Applications | FACS, IHC-P |
|---|---|
| Dilution Range | Flow Cytometry: 0.5-1ug/million cells,Immunohistology (FFPE): 0.25-0.5ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2) |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a, kappa |
| Clone No. | CDLA45RO-1 |
| Immunogen | Recombinant human protein was used as immunogen for this CD45RO antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Recombinant
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Flow cytometry testing of PFA-fixed human Jurkat cells with CD45RO antibody (clone CDLA45RO-1); Red = isotype control, Blue = CD45RO antibody.
IHC testing of FFPE human tonsil and CD45RO antibody (clone CDLA45RO-1). HIER: boil in pH6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
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UniProt Details
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
CD45RO Antibody (orb385805)
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