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CD45RO Mouse Monoclonal Antibody
SKU: orb1520122
Featured
Description
Research Area
Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 4
| Tested Applications | FC, ICC, IF, IHC, WB |
|---|---|
| Dilution Range | Flow-Cyt Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 5 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:250 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | UCHL-1 |
| Immunogen | Cultured T-cells from an IL-2-dependent T-cell line |
| Target | CD45RO |
| Purity | The antibody was purified from cell culture supernatant |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 50 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−B220; CD antigen CD45; CD45; CD45 antigen; CD45R; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen
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Detection of human CD45RO by western blot. Samples: Whole cell lysate (15 and 50 µg) from Jurkat cells prepared using NETN lysis buffer. Antibody: mouse anti-CD45RO antibody used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 minutes
Detection of human CD45RO by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD45RO antibody used at a dilution of 1:250. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: DAB. Counterstain: Hematoxylin (blue)
Detection of human CD45RO (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Mouse anti-CD45RO monoclonal antibody used at 1:250. Substrate: Opal™. Counterstain: DAPI (blue)
Detection of human CD45RO (shaded) in Jurkat cells by flow cytometry. Antibody: Mouse anti-CD45RO recombinant monoclonal or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-mouse IgG
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_563578.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
CD45RO Mouse Monoclonal Antibody (orb1520122)
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