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CD48 Antibody
SKU: orb1824372
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | Mouse IgG1, kappa |
| Clone No. | rCD48/8834 |
| Immunogen | A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the CD48 antibody. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC staining of FFPE human tonsil tissue with CD48 antibody (clone rCD48/8834). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD48 antibody (clone rCD48/8834) as confirmation of integrity and purity.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
CD48 Antibody (orb1824372)
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