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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB:1:500-1:5,000, IF:1:25-1:250, IHC-P:1:250-1:1,000, IHC-F:1:250-1:1,000 |
| Reactivity | Canine, Human, Monkey, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Antigen: Purified recombinant peptide derived from within residues 120 aa to 175 aa of human CD63 produced in E. coli. Antigen Sequence: MENYPKNNHTASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCI |
| Target | Lysosome-associated membrane glycoprotein 3 |
| Purification | Epitope affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Polyclonal antibody supplied as a 200 or 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum. |
| Concentration | Please inquire |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD63 antigen, CD63 antigen (melanoma 1 antigen), granulophysin, LAMP-3, lysosomal-associated membrane protein 3, lysosome-associated membrane glycoprotein 3, ME491, melanoma-associated antigen, melanoma 1 antigen, melanoma-associated antigen ME491, MLA1, ocular melanoma-associated antigen, OMA81H, tetraspanin-30, TSPAN30 antibody.
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Anti-CD63 Ab at 1/2,500 dilution; lysate at 50 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.
IHC of mouse spleen using anti-CD63 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD63 Ab at 1:500/DAB detection.
Immunofluorescence – anti-CD63 Ab in Hepa1-6 cells at 1/50 dilution; cells were fixed with 4% of PFA.
IHC of human pancreas using anti-CD63 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD63 Ab at 1:500/DAB detection.
Anti-CD63 Ab at 1/2,500 dilution; lysate at 50 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.
IHC of human cervix using anti-CD63 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD63 Ab at 1:500/DAB detection.
Immunofluorescence – anti-CD63 Ab in Hepa1-6 cells at 1/50 dilution; cells were fixed with 4% of PFA.
IHC of rat eye using anti-CD63 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD63 Ab at 1:500/DAB detection.
Quick Database Links
Gene Symbol
Lysosome-associated membrane glycoprotein 3
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Liu, Dishiwen et al. Cardiac Fibroblasts Promote Ferroptosis in Atrial Fibrillation by Secreting Exo-miR-23a-3p Targeting SLC7A11 Oxid Med Cell Longev, 2022, 3961495 (2022)
CD63 Antibody (orb11597)
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