CD66a Recombinant Rabbit Monoclonal Antibody

SKU: orb1152074

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Description

Research Area

Cancer Research, Tumor Biology

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, WB=1:500-2000
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	A10D5
Immunogen	A synthesized peptide derived from human CEACAM1 (480-526/526aa)
Target	CEACAM1
Molecular Weight	57 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CEACAM1; carcinoembryonic antigen-related cell adhesion molecule 1 isoform 1 precursor; Antigen CD66; BGP; BGP 1; BGP I; BGP1; BGPI; Biliary glycoprotein 1; Biliary glycoprotein adhesion molecule; Biliary glycoprotein; Carcinoembryonic antigen related cell adhesion molecule 1; CD66 antigen; CD66a antigen; CEACAM 1; bb-1; C-CAM; Cc1; Cea-1; Cea-7; Cea1; Cea7; Hv-2; Hv2; mCEA1; Mhv-1; MHVR; MHVR1; mmCGM1; mmCGM1a; mmCGM2; CEAM1_HUMAN.

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CD66a Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD66a) Monoclonal Antibody, Unconjugated (orb1152074) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD66a) Monoclonal Antibody, Unconjugated (orb1152074) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue: Human colon cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1152074.

Quick Database Links

Gene Symbol

CEACAM1

UniProt

P13688

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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