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CD7 Antibody
SKU: orb1824896
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | Rabbit IgG, kappa |
| Clone No. | CD7/3868R |
| Immunogen | Recombinant full-length human CD7 protein was used as the immunogen for the CD7 antibody. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot testing of human Jurkat cell lysate with CD7 antibody (clone CD7/3868R). Expected molecular weight: 25-40 kDa depending on glycosylation level.
IHC staining of FFPE human lymph node tissue with CD7 antibody (clone CD7/3868R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with CD7 antibody (clone CD7/3868R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney tissue with CD7 antibody (clone CD7/3868R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
CD7 Antibody (orb1824896)
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