CD71 Antibody (C-term)

Flow cytometric analysis of hela cells using CD71 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Staining CD71 in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Formalin-fixed and paraffin-embedded human brain tissue reacted with CD71 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of CD71 Antibody (C-term) in Hela cell line lysates (35 ug/lane). CD71 (arrow) was detected using the purified Pab.

All lanes: Anti-CD71 Antibody (C-term) at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: CCRF-CEM whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 85 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.