NT5E Antibody (Biotin)

SKU: orb750881

Description

Mouse monoclonal antibody against NT5E conjugated to Biotin

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Neuroscience

Images & Validation

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Item 1 of 6

Tested Applications	ELISA, FC, IF, IHC, WB
Dilution Range	ELISA (For coating, order antibody without BSA);Flow Cytometry (2-4ug/million cells in 0.1ml);,Immunofluorescence (2-4ug/ml); Western Blot (2-4ug/ml); ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Reactivity	Human
Application Notes	Positive Control: A431, U87MG or PC-3 cells. Human tonsil, prostate or lung carcinoma.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	NT5E/2646
Immunogen	Recombinant full-length human NT5E protein
Target	NT5E
Source/Expression System	Bioreactor
Molecular Weight	71kDa
Purification	Protein G
Conjugation	Biotin

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Buffer/Preservatives	200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Concentration	Purified Ab conjugated to Biotin
Disclaimer	For research use only

Alternative Names

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Anti-5&apos antibody, anti-NT antibody, anti-5&apos antibody, anti-nucleotidase (CD73) antibody, anti-5&apos antibody, anti-nucleotidase precursor antibody, anti-5&apos antibody, anti-nucleotidase antibody, anti-ecto antibody, anti-5&apos antibody, anti-nucleotidase antibody, anti-ecto (CD73) antibody, anti-E5NT antibody, anti-Ecto 5&apos antibody, anti-nucleotidase antibody, anti-Ecto-5&apos antibody, anti--nucleotidase antibody, anti-eN antibody, anti-eNT antibody, anti-NT antibody, anti-NT5 antibody, anti-NT5E antibody, anti-NTE antibody, anti-Purine 5 Prime Nucleotidase antibody

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Analysis of Protein Array containing more than 19000 full-length human proteins using CD73-Monospecific Mouse Monoclonal Antibody (CD73/2646). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Flow Cytometric Analysis of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2646) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2646).

Immunofluorescence staining of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2646) followed by goat anti-mouse IgG-CF488 (green). Membrane stained with phalloidin (red).

SDS-PAGE Analysis Purified CD73 Mouse Monoclonal Antibody (NT5E/2646). Confirmation of Integrity and Purity of Antibody.

Quick Database Links

Gene Symbol

NT5E

UniProt

P21589

NCBI Gene ID

4907

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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