Recombinant CD79a Antibody / Rabbit Monoclonal

SKU: orb534000

Description

The CD79a protein together with the related CD79b protein, forms a dimer associated with membrane-bound immunoglobulin in B-cells, thus forming the B-cell antigen receptor (BCR). CD79a plays multiple and diverse roles in B cell development and function. The CD79a/b heterodimer associates non-covalently with the immunoglobulin heavy chain through its transmembrane region, thus forming the BCR along with the immunoglobulin light chain and the pre-BCR when associated with the surrogate light chain in developing B cells. Association of the CD79a/b heterodimer with the immunoglobulin heavy chain is required for surface expression of the BCR and BCR induced calcium flux and protein tyrosine phosphorylation. [Wiki]

Research Area

Epigenetics, Immunology

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution range	Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)
Reactivity	Human
Application Notes	The stated application concentrations are suggested starting points. Titration of the CD79a antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	CDLA79a-3R
Immunogen	Amino acids 202-216 (GTYQDVGSLNIADVQ) were used as the immunogen for the recombinant CD79a antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Recombinant CD79a Antibody / Rabbit Monoclonal

Flow cytometry testing of human Raji cells with recombinant CD79a antibody (clone CDLA79a-3R); Red = isotype control, Blue = CD79a antibody.

Western blot testing of human Raji cell lysate with recombinant CD79a antibody (clone CDLA79a-3R). Expected molecular weight: 25-47 kDa depending on glycosylation level.

Immunofluorescent staining of PFA-fixed human Raji cells with recombinant CD79a antibody (green, clone CDLA79a-3R) and Reddot nuclear stain (red).

IHC testing of FFPE human tonsil with recombinant CD79a antibody (clone CDLA79a-3R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA followed by cooling at RT for 20 min.

Quick Database Links

UniProt

P11912

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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