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Description
Research Area
CD Markers, Cell Biology, Stem Cell, Stem Cells
Images & Validation
−Item 1 of 2
| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution Range | ICC/IF=1:100-500, Flow-Cyt=1ug/Test, WB=1:500-2000 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 11B12 |
| Immunogen | Recombinant mouse CD80 protein (38-246/306aa) |
| Target | CD80 |
| Molecular Weight | 47 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7; B71; Cd28l; Ly-53; Ly53; MIC17; TSA1; CD80_HUMAN; CD80; Activation B7-1 antigen; CTLA-4 counter-receptor B7.1 (B7); CD80_MOUSE; Activation B7-1 antigen (B7);
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Blank control: Raji. Primary Antibody (green line): Mouse Anti-CD80 antibody (orb1974707), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Raji cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CD80) monoclonal Antibody, Unconjugated (orb1974707) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
CD80
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry
CD80 Mouse Monoclonal Antibody (orb1974707)
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