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CD9 Antibody
SKU: orb1294290
Description
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:500-1:800 Immunofluorescence 1:300-1:400 Immunohistochemistry (Paraffin) 1:50-1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human CD9 |
| Target | CD9 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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CD9 antibody at 1/1000 dilution Lysates at 25 µg per lane.This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294290 overnight at 4°C.
CD9 antibody at 1/500 dilution Lysates at 30 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294290 overnight at 4°C.
CD9 antibody at 1/800 dilution Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294290 overnight at 4°C.
CD9 antibody at 1/800 dilution Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294290 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294290 anti-CD9 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD9 with orb1294290 at 1/100.
Quick Database Links
Gene Symbol
CD9
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
CD9 Antibody (orb1294290)
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