Cdk9 Recombinant Rabbit Monoclonal Antibody

SKU: orb559221

Description

Research Area

Defensins, Epigenetics and Nuclear Signaling, HIV, Immune System, Infectious Disease

Images & Validation

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Item 1 of 4

Tested Applications	FC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-200, IHC-F=1:100-200, IF=1:100-200, ICC/IF=1:50-200, Flow-Cyt=1:50-100
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	8C3
Immunogen	Recombinant human Cdk9 protein, full length
Target	CDK9
Molecular Weight	43 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CDK9_HUMAN; Cyclin-dependent kinase 9; EC:2.7.11.22; EC:2.7.11.23; CDK9; CDC2L4; TAK; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit;

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Cdk9 Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab, dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: 293T, 3: Raw264.7, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 43 kDa, Observed MW: 42, 55 kDa.

Tissue: Human pancreas, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab, dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559221.

Tissue: Mouse liver, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab, dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559221.

Tissue: Rat liver, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab, dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559221.

Quick Database Links

Gene Symbol

CDK9

UniProt

P50750

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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