C/EBPB Rabbit pAb Discontinued

SKU: orb374357

This product can be custom made

Description

Rabbit polyclonal antibody to CEBPB

Research Area

Epigenetics

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC-P, WB
Dilution Range	WB: 1:500-1000
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 171-216 of human C/EBPB (NP_005185.2).
Molecular Weight	48kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CEBP-beta, IL6DBP, NF-IL6, TCF5, CEBP beta, CEBPB

Quality Guarantee

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Immunohistochemistry analysis of paraffin-embedded Mouse spleen using C/EBPB Rabbit pAb (orb374357) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat kidney using C/EBPB Rabbit pAb (orb374357) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Western blot analysis of various lysates, using C/EBPB Rabbit pAb (orb374357) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.