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KO/KD
Validated
Validated
Description
Images & Validation
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Item 1 of 2
| Tested Applications | ELISA, IF, KO/KD Validated, Multiplex Assay, WB |
|---|---|
| Dilution Range | ELISA: 1:5,000 1:20,000, WB: 1:100 - 1:500 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length human CENP-Q recombinant protein. |
| Target | CENPQ |
| Purity | This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human CENP-Q protein. A BLAST analysis of the full length sequence was used to suggest partial cross-reactivity with CENP-Q based on the following percentage homologies: macaque (91%), horse (76%), bovine (74%), dog (73%), swine (71%), mouse (65%) and rat (60%). Cross-reactivity with CENP-Q from other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.15 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-CENP-Q Antibody, CenpQ, centromere protein Q, CENP Q
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CENPQ rabbit pAb Antibody [orb772130]
ELISA, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
50 μl, 100 μl
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 5-10 working days