Certolizumab pegol Antibody

SKU: orb1980481

Description

Certolizumab pegol is a PEGylated, humanized anti-TNF-α monoclonal antibody used in research. It is widely applied in preclinical studies for autoimmune diseases like rheumatoid arthritis, psoriatic arthritis, and axial spondyloarthritis, utilizing both in vitro and in vivo experimental models.

Research Area

Cell Biology

Images & Validation

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Key Properties

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CAS Number	428863-50-7
MW	47.74 kDa
Purity	95.00%
Target	TNF

Bioactivity

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Target IC50	TNF-α:3 ng/mL (IC90)
In Vivo	Acute edematous pancreatitis was induced in healthy Wistar Albino male rats by intraperitoneal injection of 80 μg/kg of Cerulein (20 μg/kg each time for 4 doses, 1 hour apart). Subsequently, a single intraperitoneal injection of 10 μg Certolizumab pegol significantly reduced serum levels of amylase, lipase and lactate dehydrogenase. Histopathologic examination showed significant reductions in edema, hemorrhage, parenchymal necrosis, and infiltration scores. In addition, malondialdehyde, myeloperoxidase, TNF-α and caspase-3 activities were significantly reduced. Read more...
In Vitro	Certolizumab pegol was able to neutralize soluble TNFα with an IC90 value of 3 ng/mL.Certolizumab pegol was able to completely inhibit IL-1β production by human monocytes in response to LPS stimulation after treatment at concentrations ranging from 0-100 μg/mL for 1 h. Certolizumab pegol does not mediate complement-dependent cytotoxicity (CDC) or antibody-dependent cell-mediated cytotoxicity (ADCC). In addition, Certolizumab pegol does not induce apoptosis in activated human monocytes or peripheral blood lymphocytes (PBL), nor does it cause degranulation or disruption of cell membrane integrity in polymorphonuclear cells. Read more...

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PHA738144, PHA-738144, TNF-α, Certolizumab, CDP8 70, CDP870

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Gene Symbol

TNF

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