CFL1 Antibody (Center)

SKU: orb1931349

Description

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 5

Tested Applications	FC, WB
Dilution range	WB - 1:1000, FC - 1:10-50
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Gallus, Monkey, Porcine, Rat, Xenopus

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	18502 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

Alternative Names

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CFL

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CFL1 Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of CFL1 Antibody (Center) in HL-60 cell line lysates (35 ug/lane). CFL1 (arrow) was detected using the purified Pab;

Western blot analysis of CFL1 Antibody (Center) in mouse NIH-3T3 cell line lysates (35 ug/lane). CFL1 (arrow) was detected using the purified Pab.

Western blot analysis of CFL1 (arrow) using rabbit polyclonal CFL1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CFL1 gene.

Anti-CFL1 Antibody (Center) at 1:1000 dilution + MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P23528

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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