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Validated
Description
Research Area
Images & Validation
−| Tested Applications | FC, IF, IHC, IP, KO/KD Validated, WB |
|---|---|
| Dilution Range | WB 1:500~1000, IHC 1:150, IF 1:50~100, FLOW 1:100, IP 2ug/500ul |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | BQVK7E6 |
| Immunogen | Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell. |
| Target | Chk2 |
| Molecular Weight | 60.7 kDa |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS (pH 7.3) containing 1% rAlbumin, 50% glycerol and 0.02% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and CHEK2-Knockout hela cells (KO) were separated by SDS-PAGE and immunoblotted using Chk2 (CHEK2) antibody
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CHEK2 Antibody (orb1325509)
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