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Validated
Description
Research Area
Images & Validation
−| Tested Applications | FC, IF, IHC, IP, KO/KD Validated, WB |
|---|---|
| Dilution Range | WB 1:500~1000, IHC 1:150, IF 1:50~100, FLOW 1:100, IP 2ug/500ul |
| Reactivity | Canine, Human, Mouse, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | BQVK7E6 |
| Immunogen | Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell. |
| Target | CHEK2 |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and CHEK2-Knockout hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-CHEK2 monoclonal antibody using Chk2 (CHEK2) antibody
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CHEK2 Antibody (orb1338524)
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