Chicken Goat IgG (H&L), HRP conjugated Antibody

SKU: orb700008

Description

Gallus polyclonal antibody against Gallus anti-Goat IgG (H&L) conjugated to HRP

Images & Validation

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Dilution range	1 : 10 000-1 : 50 000 (ELISA), 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
Reactivity	Goat
Application Notes	This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG Read more...

Key Properties

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Host	Gallus
Clonality	Polyclonal
Purification	Immunogen affinity purified IgY.
Conjugation	HRP

Storage & Handling

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Storage	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Form/Appearance	Lyophilized
Disclaimer	For research use only

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