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Chicken IgG (H&L) Antibody Biotin Conjugated
SKU: orb346896
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:98,500, IHC: 1:500 - 1:3,000, WB: 1:3,000 - 1:30,000 |
| Reactivity | Gallus |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Chicken IgG whole molecule |
| Purity | Anti-Chicken IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Chicken IgG and Chicken Serum. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 2.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−goat anti-Chicken IgG Antibody Biotin Conjugation, goat anti-Chicken IgG BAC Conjugated Antibody, goat anti-Chicken IgY Antibody Biotin Conjugation, goat anti-Chicken IgY BAC Conjugated Antibody
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ELISA results of purified Goat anti-Chicken IgG Biotin Conjugated Antibody tested against purified Chicken IgG (p/n orb2652774). Each well was coated in duplicate with 1.0 µg of Chicken IgY (blue line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Streptavidin HRP conjugate.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Chicken IgG (H&L) Antibody Biotin Conjugated (orb346896)
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