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Chicken IgG (H&L) Antibody Texas Red Conjugated Pre-Adsorbed
SKU: orb346901
Description
Images & Validation
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| Tested Applications | FC, FLISA, IF, WB |
|---|---|
| Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Reactivity | Gallus |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Chicken IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins. |
| Conjugation | Texas Red |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−goat anti-Chicken IgG Texas Red Conjugated Antibody, goat anti-Chicken IgY Antibody Texas Red Conjugation, Chicken Secondary Antibody
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Chicken IgG (H&L) Antibody Texas Red Conjugated Pre-Adsorbed [orb346931]
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Western Blot of Unconjugated Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n orb346886). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG/IgY whole molecule (p/n orb2652774). Lane 2: Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Chicken IgG Fab Fragment (p/n orb2652771). Lane 4: Chicken IgM Whole Molecule (p/n orb346054). Lane 5: Chicken Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n orb346886) 1:3000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
Chicken IgG (H&L) Antibody Texas Red Conjugated Pre-Adsorbed (orb346901)
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