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CLDN1 Recombinant Rabbit Monoclonal Antibody

SKU: orb1172770

Description

CLDN1 Recombinant Rabbit Monoclonal Antibody

Images & Validation

Tested ApplicationsWB
Dilution rangeWB=1:500-2000
ReactivityHuman, Mouse
Predicted ReactivityMouse

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG
Clone No.B5D10
ImmunogenA synthesized peptide derived from human Claudin 1 (180-211aa)
TargetCLDN1
Molecular Weight23 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

CLD1_HUMAN; Claudin-1; Claudin 1; Claudin1; CLD1; SEMP1; Senescence-associated epithelial membrane protein; UNQ481/PRO944; ILVASC;
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

CLDN1 Recombinant Rabbit Monoclonal Antibody

25 ug total protein per lane of various lysates probed with CLDN1 monoclonal antibody, unconjugated (orb1172770) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

CLDN1 Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Human HepG2 cell lysates, Primary: Anti-CLDN1 (orb1172770) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: kDa, Observed band size: 18 kDa.

CLDN1 Recombinant Rabbit Monoclonal Antibody

Tissue: Human colon cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172770.

CLDN1 Recombinant Rabbit Monoclonal Antibody

Tissue: Human esophagus, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172770.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol

CLDN1 Recombinant Rabbit Monoclonal Antibody (orb1172770)

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Available Sizes

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25 μl
$ 170.00
50 μl
$ 320.00
100 μl
$ 460.00
DispatchUsually dispatched within 5-10 working days
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