Cleaved PARP Recombinant Rabbit Monoclonal Antibody

SKU: orb704341

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Description

Research Area

ADP-ribosylation, Cell Signaling, DNA / RNA, DNA Repair, DNA and RNA Research, Epigenetics and Nuclear Signaling

Images & Validation

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Item 1 of 3

Tested Applications	FC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, Flow-Cyt=1:50-100, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	2A10
Immunogen	A synthesized peptide derived from human PARP 1 (214-250aa)
Target	PARP1
Molecular Weight	89/113 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ADP ribosyltransferase(NAD+; poly(ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP1; PARP-1; Poly(ADP ribose) polymerase 1; poly(ADP ribose) polymerase family, member 1; Poly adenosine diphosphate ADP ribose polymerase; Poly ADP ribose polymerase 1; Poly ADP ribose polymerase family member 1; Poly ADP ribose synthetase 1; poly(ADP ribose) synthetase; poly(ADP ribosyl)transferase; Poly[ADP ribose] synthetase 1; PPOL; sPARP 1; sPARP1; PARP1_HUMAN.

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Cleaved PARP Recombinant Rabbit Monoclonal Antibody

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Cleaved PARP antibody (orb704341), dilution: 1:50, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Human Jurkat cell lysates, Lane 2: Human THP-1 cell lysates, Lane 3: Human HL60 cell lysates, Lane 4: Human K562 cell lysates, Primary: Anti-Cleaved PARP (orb704341) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 89/116 kDa, Observed band size: 100 kDa.

Sample: Lane 1: Jurkat cell lysate, Lane 2: A549 cell lysate, Primary: Anti-Cleaved PARP (orb704341) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 89/110 kD, Observed band size: 89 kD.

Quick Database Links

Gene Symbol

PARP1

UniProt

P09874

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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