CLEC5A Polyclonal Antibody

SKU: orb1417590

Description

Rabbit polyclonal antibody to CLEC5A.

Research Area

Epigenetics, Immunology

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, ICC, IF, IHC-Fr, IHC-P, WB
Reactivity	Porcine, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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C-type lectin domain family 5 member A, C-type lectin superfamily member 5, Myeloid DAP12-associating lectin 1, MDL-1, Clec5a, Clecsf5, Mdl1

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Sample: Spleen (Mouse) Lysate at 40 ug. Primary: Anti-CLEC5A at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 22 kD. Observed band size: 22 kD.

Cell: U937. Concentration: 1: 100. Incubation: 40 minutes, room temperature.Host/Blank: U937 cells.Flow cytometric analysis of Rabbit Anti-CLEC5A antibody (green) compared with control in the absence of primary antibody (blue) followed by U937 cells.secondary antibody: Goat Anti-rabbit IgG/FITC antibody.

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CLEC5A/MDL1 Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

UniProt

Q9R007

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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