CLEC9A Antibody / DNGR-1

SKU: orb640076

Description

This mAb recognizes mouse DNGR-1, which is also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognize a preformed signal exposed on necrotic cells. In peripheral blood, it is highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.

Images & Validation

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Tested Applications	FACS, IF, WB
Dilution range	Flow cytometry: 1-2ug/10^6 cells in 0.1ml,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml
Reactivity	Mouse
Application Notes	Optimal dilution of the CLEC9A antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rat
Clonality	Monoclonal
Isotype	Rat IgG1, kappa
Clone No.	1F6
Immunogen	RBL-2H3 cells expressing mouse CLEC9A fused to an HA epitope.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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SDS-PAGE analysis of purified, BSA-free CLEC9A antibody (clone 1F6) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q8RU4

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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