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Col003

SKU: orb1309817

Description

Col003 is a potent and selective Hsp47 inhibitor that competitively targets its collagen-binding site with an IC50 of 1.8 µM. This small molecule is a valuable research tool for studying fibrosis mechanisms in both cellular and animal models.

Research Area

Metabolism Research, Signal Transduction

Images & Validation

Key Properties

CAS Number328565-16-8
MW257.24
Purity99.96% (May vary between batches)
FormulaC14H11NO4
SMILESOc1c(C=O)cc(Cc2ccccc2)cc1[N+]([O-])=O
TargetHSP
SolubilityDMSO:188 mg/mL (730.84 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.77 mM)

Bioactivity

Target IC50
HSP47:1.8 μM
In Vitro
A molecule AK778 and its cleavage product Col003 competitively inhibited the interaction and caused the inhibition of collagen secretion by destabilizing the collagen triple helix. Structural information obtained with NMR analysis revealed that Col003 competitively binds to the collagen-binding site on Hsp47. Col003 has an inhibitory effect on the interaction of Hsp47 with collagen in Hsp47 KO / MEFs. Col003 (0.01-100 μM) exhibits dose-dependent effects on the interactions of Hsp47 with collagen (IC50: 1.8 μM for Hsp47). Col003 (100 μM; 20-60 mins) has an inhibitory effect on collagen secretion and accumulation. It inhibits collagen secretion by wild-type MEFs and the secretion is not abolished completely. Col003 (100 μM) degrades α,α′-dipyridyl collagen secretion completely by incubation with trypsin at a high temperature (50 °C for 15 min), but is resistant to trypsin digestion at 37 °C.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

structure, triple, Col 003, Col003, Col-003, collagen, Hsp47, HSP47, HSP, Inhibitor, interaction, Heat shock proteins, helix, MEF, inhibit, secretion
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Key Properties

No computed properties available.

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Protocol Information

Col003 (orb1309817)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

2 mg
$ 80.00
1 ml x 10 mM (in DMSO)
$ 110.00
5 mg
$ 110.00
10 mg
$ 150.00
25 mg
$ 270.00
50 mg
$ 410.00
100 mg
$ 620.00
200 mg
$ 860.00
500 mg
$ 1,270.00
DispatchUsually dispatched within 3-5 working days
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