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COL17A1 rabbit pAb Antibody
SKU: orb771425
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Description
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−Item 1 of 4
| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution range | WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human COL17A1. AA range:481-530 |
| Molecular Weight | 150 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-COL17A1 BP180 BPAG2 antibody
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Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
Western Blot analysis of 3T3, hepg2 cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000.
Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
COL17A1 rabbit pAb Antibody (orb771425)
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