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COL1A1/Collagen I Antibody
SKU: orb1294293
Description
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:1000-1:1500 Immunofluorescence 1:300–1:500 Immunohistochemistry (Paraffin) 1:100–1:300 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
| Target | COL1A1 / Collagen I |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Anti-COL1A1 / Collagen I antibody - at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min. Cells were stained with orb1294293 anti-COL1A1 / Collagen I antibody (red) at 1:400 and 4°C.
COL1A1 / Collagen I antibody - at 1/1000 dilution. Lysates at 40 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.
COL1A1 / Collagen I antibody - at 1/250 dilution. Lysates at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1 / Collagen I with orb1294293 at 1/150.
Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1 / Collagen I with orb1294293 at 1/150.
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling COL1A1 / Collagen I with orb1294293 at 1/100.
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling COL1A1 / Collagen I with orb1294293 at 1/100.
Quick Database Links
Gene Symbol
COL1A1 / Collagen I
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
COL1A1/Collagen I Antibody (orb1294293)
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