COL1A1/Collagen I Alpha 1 Antibody

SKU: orb1538677

Description

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC-P, WB
Dilution range	ELISA, IHC-P (5 µg/ml), WB
Reactivity	Human, Mouse
Application Notes	Further information: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG3,k
Clone No.	3G3
Immunogen	Amino acids 1021-1108 of NP_000079.1
Target	COL1A1 / Collagen I Alpha 1
Purification	Purified from ascites by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS, pH 7.4. PBS, pH 7.4
Concentration	0.48 mg/ml
Disclaimer	For research use only

Alternative Names

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COL1A1, Alpha-1 type I collagen, Collagen alpha 1 chain type I, Collagen alpha-1(I) chain, Collagen, type I, alpha 1, Collagen I, Pro-alpha-1 collagen type 1, OI4

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Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

COL1A1 monoclonal antibody clone 3G3. Western blot of COL1A1 expression in NIH/3T3.

Detection limit for recombinant GST tagged COL1A1 is 0.03 ng/ml as a capture antibody.

Quick Database Links

Gene Symbol

COL1A1 / Collagen I Alpha 1

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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